The three-year-old campaign leading up to the 1000 and 2000 Guineas in April reaches a crucial stage at Caymanas Park today with the $1.3 million Thornbird Stakes for native-bred fillies and the Prince Consort Stakes for colts and geldings, both sponsored by Front Runner, sharing the spotlight over 1400 metres. The fillies, in particular, have been lighting up the campaign, and going into this their final prep, the 11 declared coming to ensure an intriguing contest. Among the principals are the hat-trick seekers RUNALLDAY from the Anthony ‘Baba’ Nunes stables, BILINGUAL, who is also trained by Nunes, and Dwight Chen’s MARLENE MY LOVE, All three have looked potential classic winners but may need to show further improvement to stop the classy SHE’S A MANEATER, who has won four races from five carer starts, including a fast-time win over this trip in January. Trained by 16-time champion Wayne DaCosta for owner Winston Kong, the free-running bay filly by Natural Selection out of the 2009 ‘Horse of the Year’ Ahwhofah lost her only race on Boxing Day when caught and beaten by the grey colt SOTOMAYOR in the $4..25 million Supreme Ventures Jamaica 2-Y-O Stakes over a mile, a race in which she turned for home a clear leader. However, she showed in her last race that she had definitely trained on, winning impressively ahead of subsequent winner ANOTHER FURY in the good time of 1;25.3 with customary rider, reigning champion jockey Omar Walker aboard. She continues to look sharp at exercise, and having won twice over this trip, her proven class should ensure that nothing overhauls her in the closing stages. Three time winner DaCosta and Walker should complete the feature race double with recent winner FEARLESS SAMURAI in the Prince Consort Stakes to be contested by nine starters. The chestnut colt by Fearless Vision out of Sayuri has won three races from five starts, but really turned heads last time out when slamming MONEY MAGNET over 1100 metres, covering the distance in the smart time of 1:06.0. FEARLESS SAMURAI (working well) will be seen to even better advantage over this trip, and despite the strong claims of hat-trick seeker DON CHASSIS and recent winner DONTAE, he should take a lot of catching. A notable absentee from the line-up is the ‘Baba’ Nunes-trained SOTOMAYOR, who has bypassed the Prince Consort Stakes in preference for the 3-y-o and up overnight allowance mile, a race he should win ahead of the in-form IAN LINKS, now stepping up to a mile. Other firm fancies on the card are LAWS OF THE CODE in the first race, ZEPHYR in the third, BIG BLACK NATION in the fourth, TIZ A VIBE in the fifth and SUBBIE in the seventh.
Dear Editor,There is a direct relationship between the operations of a Government of any country, and its functions to influence governance approaches that are binding on citizens, partnerships with other States, as well as national and international business arrangements.The foundation of such arrangements is enshrined in the Constitution of a State, as it informs the basis under which current and future behavioral norms of any Government is premised. Unconstitutionality in this realm is therefore intolerable!The consequences for breaches of these central Constitutional pillars will not only undermine the current relationships and existing obligations of the State, but will be dire for all futuristic arrangements as it informs a defining framework of untrustworthiness and uncertainty. Such an unpredictable environment feeds illegitimacy, and fuels circumstances under which only the few in Government can benefit.Under the globally accepted democratic formula to which Guyana is constitutionally committed, things have become glaringly abnormal. There is now significant apprehension nationally and internationally, given the surreptitious delaying signals from the fallen of the APNU/AFC Government in response to the passing of the historic No-Confidence Motion in the Nationally Assembly on December 21, 2018.Today, imminent personalities and prominent Organisations both nationally and internationally, are justifiably demanding the activation of the mechanism for the holding of General and Regional Elections within ninety days as instructed by the Guyana Constitution. The immediate resignation of the Cabinet prescribed by Section 106 of the Constitution implies and infers a meaning of immediate suspension of forward functionality by the Principals of this Government Organ. The President is also committed to resign his individual portfolio on the election of a President following elections held in the prescribed period.It is implicit that during this period, Ministers, Prime Minister as well as the persons who formed the Cabinet, would have lost their portfolio. We should refer to them by their respective names only! These rude, abusive and disrespectful persons must face the citizens at the ensuing polls and be held accountable for their activities that informed the loss of confidence by the majority of citizens.Amidst the high tension and the debacle of our citizens’ exposure to the significant incompetence of this APNU/AFC cabal, it is indeed very heartening to see that Guyanese are responding to this present political situation very sensibly.Observably, our people are demonstrating the will to charter their own destiny, and not leave it in the hands of a few self-aggrandising and uncaring individuals. Citizens are going about their daily lives with cool, and waiting for the constitutionally instructive period to change this trespassing APNU/AFC Government by March 19, 2019. Guyanese must not allow our country to have an unconstitutional Government and our citizens have issued the eviction notice.Sincerely,Neil Kumar
Explore further (PhysOrg.com) — With its unique double-helical structure, DNA has the ability to be used as a programmable building material to construct designer nanoscale architectures. Complex DNA architectures could have a variety of applications, from DNA-based nanomotors to biosensing and drug delivery. Taking the research a step forward, researchers have recently constructed a nanometer-sized tetrahedron from a single strand of DNA, using a method that could have advantages for assembling similar structures on a large scale. Front and top views of the 3D molecular model of the tetrahedron. Image copyright: Zhe Li, et al. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. The researchers, from Arizona State University (ASU) and the Hong Kong University of Science and Technology (HKUST), have published their results in a recent issue of the Journal of the American Chemical Society. As the researchers explain, the variety of different artificial DNA constructions has been increasing. So far, 3D DNA nanostructures are made from multiple DNA strands (oligonucleotides) with deliberately designed sequences. In this new study, Hao Yan of ASU, Yongli Mi of HKUST, and their colleagues have shown that DNA tetrahedrons can now be self-folded from only a single DNA strand. In addition, they demonstrated a method to replicate the DNA tetrahedrons in vivo, which could also be applied to the design and replication of other DNA nanostructures in the future.“A self-folded 3D nanocage that can be replicated in vivo tells us how powerful nature’s machineries are,” Yan and Mi told PhysOrg.com. “DNA nanostructures can serve as scaffolds to organize other material with controlled spatial arrangement. Spatial dependent biomolecular/nanomaterial interactions can thus be tuned and studied.”The DNA tetrahedrons, made of four triangular faces, were constructed from a DNA strand that was 286 nucleotides long. The tetrahedron’s six edges were composed of double helices: five were identical (double helical), while the sixth edge had a more complex “twin double-helical” structure. Four of the edges contained a cleavable site in the center, and all four vertices consisted of an unpaired thymine base to allow adequate flexibility for folding at these corners. Once the DNA strand was paired in this way, the researchers annealed the DNA in a process of heating and then cooling. When annealed, the DNA strand self-assembled into the seven-nanometer-long tetrahedron shape by combining the appropriate base pairs together. After confirming the successful assembly of the DNA tetrahedron, the researchers then developed a method to replicate the nanostructures using in vivo cloning in order to produce the nanostructures on a large scale. The researchers inserted one of the tetrahedrons into a cloning molecule called a phagemid, and then recovered several replicated tetrahedrons through a process of restriction digestion of the phagemid. This method is fully scalable, with the yield of cloned structures proportional to the size of the culture medium. As the researchers explain, using only a single DNA strand for creating nanostructures has several advantages, including simplifying the assembly process, increasing yield, offering the ability to scale up production, and creating structures with longer life spans in biological systems, such as inside living cells. This property is especially appealing for in vivo applications such as biosensing and drug delivery. In the future, the researchers hope to build on this method to synthesize nanostructures out of RNA, as well as build other complex shapes.More information: Zhe Li, Bryan Wei, Jeanette Nangreave, Chenxiang Lin, Yan Liu, Yongli Mi, and Hao Yan. “A Replicable Tetrahedral Nanostructure Self-Assembled from a Single DNA Strand.” J. Am. Chem. Soc. Doi: 10.1021/ja903768fCopyright 2009 PhysOrg.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed in whole or part without the express written permission of PhysOrg.com. Citation: Scientists Build Nanostructures out of Single DNA Strands (2009, September 8) retrieved 18 August 2019 from https://phys.org/news/2009-09-scientists-nanostructures-dna-strands.html Using living cells as nanotechnology factories